Frozen Storage, Culture and Transfer of Bovine Embryos
نویسندگان
چکیده
منابع مشابه
Developmental competence of bovine embryos reconstructed by the transfer of somatic cells derived from frozen tissues.
The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -35 degrees C or -80 degrees C in the presence of 5% DMSO were used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstructed from the somatic cells, 78% to 81% s...
متن کاملStorage of bovine semen in liquid and frozen state.
This review describes the historical and current methods used for storage of bovine semen. The essential physiological differences between liquid and frozen semen, their relative advantages and disadvantages are addressed, and the current state of technology, the procedures used, their merits and future possibilities are also discussed.
متن کاملO-23: Transfer of Blastocysts Derived from Frozen Thawed Cleavage Stage Embryos Improved Ongoing Pregnancy
Background: The aim of our study was to compare the resulting from transfer of embryos that cryopereserved in cleavage stage after thawing and transfer of embryos after thawing and culture in sequentional media until blastocyst formation. Materials and Methods: In this prospective clinical study, we have evaluated 128 cycles in women undergone in vitro fertilization (IVF) or intracytoplasmic sp...
متن کاملHuman pregnancy after transfer of intact frozen-thawed embryos.
Since the birth of the first baby as a result of in vitro fertilisation (IVF) in 1978, many clinics around the world have achieved pregnancies and births for their patients by using IVF and gamete intrafallopian transfer procedures. With the storage of excess embryos, multiple laparoscopies can be avoided; this has favoured the development of better cryopreservation techniques. In our clinic 8-...
متن کاملReduced pregnancy rates following the transfer of human embryos frozen or thawed in culture media supplemented with normal serum albumin.
Over a 26 month period 17% of couples having treatment in our clinical programmes selected a commercially available protein (normal serum albumin, NSA) prepared from pooled human sera instead of using their own serum as a supplement for their embryo culture media. In a retrospective analysis of >2000 gonadotrophin-stimulated cycles and 1000 cycles where frozen/thawed embryos were transferred, f...
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ژورنال
عنوان ژورنال: Journal of the Japan Veterinary Medical Association
سال: 1983
ISSN: 0446-6454,2186-0211
DOI: 10.12935/jvma1951.36.643